BDB -BiopanningDataSet Details

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BDB Biopanning Data Bank | Home

Formerly known as MimoDB

An Information Portal to Biopanning Data

As of Wednesday Jan 01, 2025 there are 3682 sets of biopanning data | BDB Statistics |

BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1775

Peptides:

Sequences
GDWVFI(6) GDWAFI(1) GDFRFT(1) GNFVFI(1) VDWAFI(1) PWPWLG(4) PWLWLQ(1) PWDWLY(1) AFWAWL(2) TWWGYL(1)

Sequences Features:

Motif:

Unique Sequence Number:

9

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

2

Reference:

Target Name:

Streptavidin

Template Name:

Biotin

Structure of Target-Template Complex:

1DF8

View the interaction!

1LUQ

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1MEP

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1MK5

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1N43

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1N9M

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1NDJ

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1NQM

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1STP

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1SWD

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1SWE

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1SWG

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1SWK

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1SWN

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1SWP

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1SWR

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1SWT

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2F01

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2GH7

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2IZF

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2IZG

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2IZH

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2IZI

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2IZJ

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2RTD

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2RTE

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2RTF

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2RTG

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2Y3F

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3MG5

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Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-6mer phage display library (X6)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Only phage displaying the GDWVFI and PWPWLG peptides demonstrated biotin-sensitive streptavidin binding in ELISA and micropanning assays. Binding of streptavidin to phage displaying GDWVFI was also confirmed by a BIACORE analysis; both biotin and synthetic HPQ peptide [YGGHPQGG] blocked this binding. Phage displaying GDWVFI could be eluted from streptavidin with either acid or biotin, but not phosphate-buffered saline, whereas phage displaying PWPWLG eluted with acid, biotin and phosphate-buffered saline. The possibility that PWPWLG sticks nonspecifically to plastic was considered; however, it was found that PWPWLG did not bind well unless wells were coated with streptavidin.

Curator:

Curation Date:

0000-00-00

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