BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1697

Peptides:

Sequences

PKCGWMYYPECKV(1)
LRCGWMYFEECKE(1)
LICGWMYFEECDS(1)
IYCGWMYYVECAY(1)
MRCGWMYFPECLN(1)
WVCHPMWEVMCLR(1)
LVCHPQGPTWCIE(1)
SWCHPQGMRECDW(1)
VGCHPMAPLWCED(1)
LWCHPQFLTTCHV(1)
NQCHPQFQGWCGM(1)
EWCHPQFNFLCGA(1)
GTCWDHPQVGCNW(1)

Sequences Features:

Motif:

H-P-[QM], C-G-W-M-Y-[FY]-x-E-C

Unique Sequence Number:

Experimental Method:

Bacterial display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16600968

Target Name:

Streptavidin

Template Name:

Biotin

Structure of Target-Template Complex:

1DF8

1LUQ

1MEP

1MK5

1N43

1N9M

1NDJ

1NQM

1STP

1SWD

1SWE

1SWG

1SWK

1SWN

1SWP

1SWR

1SWT

2F01

2GH7

2IZF

2IZG

2IZH

2IZI

2IZJ

2RTD

2RTE

2RTF

2RTG

2Y3F

3MG5

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX7(2)C bacterial display library ( X(2)-C-X(7)-C-X(2) )

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Streptavidin binding peptides were enriched using two cycles of magnetic selection (MACS) with streptavidin-coated magnetic beads. Subsequently, the enriched population was screened using two cycles of FACS. In the final round of FACS, biotin was used as a competitor to favor the detection and sorting of clones with slow dissociation kinetics.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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