BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1690

Peptides:

Sequences
NPFCSWYRWRNWCTK(1) RHLYCWTWRWCHFKD(1) SYISTWLNFLFCGQS(1) NNYSAWLRCLLRAYS(1)

Sequences Features:

Motif:

C-x-W-x(2)-W-R-x-W, S-x-W-L-x(2)-L-x(4)-S

Unique Sequence Number:

Experimental Method:

Bacterial display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 15531628

Target Name:

Human serum albumin

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 E. coli bacterial display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For negative selection, 150ml of streptavidin-coated magnetic beads (QIAgen) were incubated with cells. For positive selection, biotinylated antigen (typically 1-100 nM) was added to the supernatant fraction and incubated on ice for 30-60 min. Cells were centrifuged as above and resuspended in 7.5 ml of cold PBS with 150ml of streptavidin-coated magnetic particles (QIAgen or Miltenyi). Consensus sequences were readily apparent for the target protein after two to three rounds of magnetic selection and one or two rounds of FACS.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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