BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1483

Peptides:

Sequences

RRTPDPAVSPWQLTY
RDGQRLTSSKTMLPY
KLTSSLRYNSPPLCF
KSTSSLRDNSPPVCF
KLTSSLRCNCPPLRF
THDLSSRASSSLSYN
QAPRLMSSLSYFPQS
DHRSPPWLTSLLTIS
DTWPTARLTSSMQYI
HTYTSHLRYVPPISL
GHRYTSSVSLTEACP
SGTSHSASTTSKWFL
NLLSVAPFWPLNDSL
SDPDQWPFWRANEYG

Sequences Features:

Motif:

R-L-T-S(2)-L-R-Y-N-P

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11157855

Target Name:

Anti-dsDNA polyclonal antibody

Template Name:

Deoxyribonucleic acid, DNA

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f88-15mer phage display library (X15)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Three rounds of screening were performed with the biotinylated anti-dsDNA antibody. For the first round of selection, 10μg of biotinylated anti-dsDNA antibodies in 400 μl of 0.5×TBST (25 mM Tris, 75 mM NaCl and 0.05% Tween 20, pH 7.5) containing 1 mg/ml dialyzed BSA were immobilized onto a streptavidin-coated Petri dish. In the subsequent rounds of selection, 1μg of biotinylayed anti-dsNDA antibodies were used.

BiopanningDataSet Comments:

Synthesized peptide RLTSSLRYNP could be recognized by 88% (37 out of 42) of anti-dsDNA antibody-positive SLE sera, suggesting that the mimotope is shared by ds and ssDNAs as well as native RNA, whereas denatured RNA was not observed to inhibit the binding. The peptide was also to elicit an immune response in rabbits and the anti-peptide rabbit serum was observed to cross-react with the peptide, ss and dsDNAs, and ss and dsDNAs could inhibit the binding of the anti-peptide serum and the peptide. However, the inhibition was not obtained with RNA.

Curator:

Curation Date:

0000-00-00

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