BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1445

Peptides:

Sequences

Affinity Values

FNWSLDYLREYH(6)
ISTVPNYLRHFH(6)
DKYRIVIARGCV(5)
LCHPQNCHTKSL(3)
DPYKLPNRRCCI(3)
YTIADLITRCCV(2)
PRDTNTIRRCCV(2)
PYYNRHHPQDNR(2)
WGPYPEWLRFYH(2)
MPDLTTHPQFAR(1)
RWIDATSHPQFD(1)
HHPMDNRSYRWP(1)
GVLHPFHHPGNV(1)
VVFRPHYLSGFH(1)
NYHHLRDLRSYH(1)
HMASWLRFFHGP(1)
DDFRFTLPRCCV(1)
DRILAIGYRCCV(1)
MHVETALVRCCI(1)
PDRLRSPMRCCI(1)
DVGFIDRNWCCV(1)

[0.537]
[0.536]
[0.544]
[0.547]
[0.603]
[0.598]
[0.584]
[0.556]
[0.552]
[0.553]
[0.533]
[0.522]
[0.530]
[0.557]
[0.532]
[0.529]
[0.612]
[0.561]
[0.543]
[0.532]
[0.360]

Sequences Features:

Motif:

H-P-[QM], [YW]-L-R-x-[YF]-H, R-C(2)-[VI]

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16819727

Target Name:

Streptavidin

Template Name:

Biotin

Structure of Target-Template Complex:

1DF8

1LUQ

1MEP

1MK5

1N43

1N9M

1NDJ

1NQM

1STP

1SWD

1SWE

1SWG

1SWK

1SWN

1SWP

1SWR

1SWT

2F01

2GH7

2IZF

2IZG

2IZH

2IZI

2IZJ

2RTD

2RTE

2RTF

2RTG

2Y3F

3MG5

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X12 T7 phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

After three rounds of biopanning, approximately 1.0e11 PFU of amplified phage pools isolated from each round of screening were analyzed for SA binding in a polyclonal (heterogeneous phage populations) phage ELISA. Absorbances at 650 nm were determined and shown. The absorbance of the phage T7 10-3b as negative control was 0.050.

Brief Description:

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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