BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1324

Peptides:

Sequences
WGRFWGRWLA(5) VCDWWGWGIC(3) YWMGWKWEGE(6) WWDFLQGSER(1) FAMWYPLGWR(1)

Sequences Features:

Motif:

W-x(2)-W

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9492279

Target Name:

Doxorubicin

Template Name:

P-glycoprotein

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

fUSE5-based X10 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To ensure a high level of the drug absorption on polysterene, doxorubicin was conjugated to BSA, and the conjugate was immobilized on polystyrene ELISA plates. Displacement with verapamil, a competitive inhibitor of doxorubicin binding with P-glycoprotein were used to elute the phages that bound to the doxorubicin-BSA2-coated plates.

BiopanningDataSet Comments:

The displacement assay showed that the phages expressing these peptides bound MDR type drugs (vinblastine, doxorubicin, verapamil, and genistein) with the same selectivity as P-glycoprotein and did not interact with non-MDR type drugs, such as arabinosylcytosine (Ara-C) and melphalan. The structure modeling suggested that all the selected sequences contained a hydrophobic envelope in which MDR drugs could be docked with substantial energy minimization.

Curator:

Curation Date:

0000-00-00

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