BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1267

Peptides:

Sequences
AHPNTAPIHPKF(2) NNQSPILKLSIH(5) WQPVNNAGAILM(1) ATPQNNMMQAQW(1)

Sequences Features:

Motif:

N(2)-x(2)-P-I-L

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20434854

Target Name:

Intact oocytes surrounded by ZP proteins

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To remove phage clones that bind to the plastic material of test tubes, the primary library was added to an empty test tube prior to incubation with oocytes.

BiopanningDataSet Comments:

NNQSPILKLSIH peptide stimulated production of anti-peptide antibodies. These antibodies bind to the acrosomal region of the canine sperm cell, demonstrating ability to act as sperm antibodies.

Curator:

Curation Date:

0000-00-00

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