BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1266

Peptides:

Sequences
CLNSFLRSC CLSTALRSC CSSWYRGAC CTGTSTRAC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20434854

Target Name:

Intact oocytes surrounded by ZP proteins

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To remove phage clones that bind to the plastic material of test tubes, the primary library was added to an empty test tube prior to incubation with oocytes.

BiopanningDataSet Comments:

Thirty-two random phage clones were analyzed after the fourth round of selection and each clone was sequenced for phage DNA. Sequencing led to identification of three dominant groups of peptide sequences [CLNSFLRSC, CVLSTALRSC (37.5%); CSSWYRGAC (22%); CTGTSTRAC (9%)]. An additional group of peptides represented by only one sequence each (most likely background clones) was isolated as well.

Curator:

Curation Date:

0000-00-00

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