BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1240

Peptides:

Sequences
VPLYSNTLRYGF SYLTTLRYGNMS TQQSVFSTTLMY IPLPPPSRPFFK QPVDMPYFRTHP

Sequences Features:

Motif:

TLRYG

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20639454

Target Name:

Anti-ARD1A polyclonal antibody

Template Name:

N-alpha-acetyltransferase 10, NatA catalytic subunit

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

During each round, the library was preincubated with IgGs purified from normal serum pooled from five healthy volunteers and immobilized on Protein G Sepharose 4 Fast Flow (GE Health care, Uppsala, Sweden) to remove nonspecific binding clones. After the preclearing step, the phage library was selected onto the pool of IgGs purified from patient serum.

BiopanningDataSet Comments:

After four rounds of selection, roughly 1% (10/1000) of phage clones exhibited binding activity to serum from patients with colon cancer. DNA from 10 positive clones that specifically reacted with serum from patients was sequenced. Among 10 phage clones examined, five types of peptides were obtained. The peptide VPLYSNTLRYGF showed significant homology to the residues 121-126 (LYSNTL) of ARD1A by BLAST homology search. Authors revealed that human arrest defective 1 (ARD1A) may serve as an indicator of unfavorable prognosis in colon cancer.

Curator:

Curation Date:

0000-00-00

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