BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1206

Peptides:

Sequences	Affinity Values
LSFAAEGEF(1) YKLTCSSAQ(1) ARLSSAVVK(1) GHSLCSCIP(1) GSRFNKVPS(1) KKKTVLPDL(1) LKIGDFPAG(1) SRRTLMSGA(1) TKIRNVAGM(1)	[NT] [NT] [-] [-] [-] [-] [-] [-] [-]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9693968

Target Name:

Anti-chicken GAD monoclonal antibody GAD1

Template Name:

Glutamate decarboxylase 67

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pVIII-9aa phage display library (X9)

Affinity Measurement Method:

Affinity Measurement Description:

Phagotopes selected by biopanning with the mAbs were tested by ELISA for reactivity with GAD1. The negative control was wild type fl bacteriophage (wt fl). NT represents not tested.

Brief Description:

After the first round of positive selection, there was a negative selection step in which phage not specifically reactive with the monoclonal antibody used for selection were removed using magnetic beads coated with anti-mouse immunoglobulin.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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