BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1205

Peptides:

Sequences	Affinity Values
CIAPKRHNSAC CESAQRPFGCC	[++] [++]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9693968

Target Name:

Anti-collagen alpha-1(II) chain monoclonal antibody CII-C1

Template Name:

Collagen alpha-1(II) chain

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pVIII-9aa.Cys phage display library (CX9C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Phagotopes selected by biopanning with the mAbs were tested by ELISA for reactivity with CII-C1. The negative control was wild type fl bacteriophage (wt fl).

Brief Description:

After the first round of positive selection, there was a negative selection step in which phage not specifically reactive with the monoclonal antibody used for selection were removed using magnetic beads coated with anti-mouse immunoglobulin.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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