BDB - Library Details

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Library

Library Name: Novagen T7 X7 phage display library

Library ID:

526

Library Length:

Library Complexity:

Library Titer:

Library From:

William C. Sessa1(Vascular Biology and Therapeutics Program and Department of Pharmacology, Yale University School of Medicine, New Haven, USA)

Library Randomness:

Library Scheme:

Library Topology:

Linear

Comments:

Novagen T7 select phage display system was used for the random screening of peptides that facilitate EC uptake in conjunction with a pool of oligonucleotides randomly coding for 7-mer peptides. For amplification, the library was inoculated with BL21 culture (OD600 of 0.5–1.0) and induced with 1 mM IPTG at 37°C for 2 hours until cell lysiswas observed. The lysate containing phages was clarified by centrifugation at 8000 g for 10 minutes, the supernatant was titered, and aliquots were stored a 4°C.

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