BDB - Library Details

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Target
Template
Library
Structure

Library

Library Name: CX9C phage display library

Library ID:

521

Library Length:

Library Complexity:

Library Titer:

Library From:

Sandip M. Kanse (Oslo University Hospital and Medical Faculty, University of Oslo, 0372 Oslo, Norway)

Library Randomness:

Library Scheme:

Library Topology:

Comments:

A library template phagemid was prepared by inserting a short oligo containing two consecutive stop codons (ocher/opal) into the NcoI/BamHI expression cassette of the pGALD9ΔL phagemid using standard methods. Using this template, a Cys-constrained random 9-mer (NNKC9) peptide library fused to pIX was constructed based on Kunkel mutagenesis essentially as described. The final E. coli SS320 transformation frequency-based library sizes were about 1.0e10 unique clones, and the libraries were prepared with high-valence peptide display by rescue with DeltaPhage.

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