BDB - Library Details

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Template
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Structure

Library

Library Name: pVIII-12aa phage display library (X12)

Library ID:

516

Library Length:

Library Complexity:

Library Titer:

Library From:

Franco Felici (Istituto di Ricerche di Biologia Molecolare, Rome, Italy)

Library Randomness:

Completely random

Library Scheme:

NNN

Library Topology:

Linear

Comments:

The phage M13 library was used, kindly donated by Prof. Franco Felici, expressing random peptides exposed on the pVIII protein, based on the phagemid vector pC8970 in which random oligonucleotide sequences were inserted in the region 5′ of the VIII gene present in the vector, under the control of the LacZ promoter. The digestion with the restriction enzymes EcoRI and BamHI linearized the vector and allowed the insertion of the oligonucleotides with random sequences, which flanked by the same restriction sites allow the recircularization of the vector through ligase reaction.

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