BDB - Library Details

Library

Library Name: TN8/6 phage display library

Library ID:

509

Library Length:

Library Complexity:

1.7e8

Library Titer:

Library From:

Dyax Corp

Library Randomness:

Semi-random

Library Scheme:

Library Topology:

Circular

Comments:

The TN8/6 phage library was constructed in the previously described M13mp18 phage derivative, MANP. The variegated parts were synthesized with TRIM technology, which incorporates trinucleotides and allows mixtures of any set of amino acid types in any desired proportions. The varied DNA sequence positions of TN8/6 phage library is depicted as "X4X2CX5X5X5X5X5X5CX2X4", where X4 = ADHR, X2 = ADFGHLPRS, X5 = ADFGHLNPQRSVW. The use of TRIM allows addition of preformed trinucleotides to the growing synthetic DNA to express the desired specification of amino acid residue distributions at each varied position. The library DNA was purified, and ligated between the NcoI and PstI sites in the prepared MANP vector. The ligated DNA products were used to transform cells of the Escherichia coli strain XL1-Blue (Stratagene).

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