BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: T7 X12, X16 and X20 phage display library pool

Library ID:

484

Library Length:

12, 16, 20

Library Complexity:

Library Titer:

Library From:

Taiji Asami (Pharmaceutical Research Division, Takeda Pharmaceutical Company, Ltd., Fujisawa)

Library Randomness:

Completely random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

To generate T7 phage libraries displaying random peptides, X12, X16, X20 (X is a mixture of twenty natural amino acids) mixed-oligonucleotides as template DNA were internally constructed, purified with a QIAquick PCR Purification kit (QIAGEN, Hilden, Germany), and ligated into the T7Select 10-3 vector (Merck Millipore, Darmstadt, Germany), according to the manufacturer's manual. The total library diversity was estimated to be 3.1e9 plaque-forming units (pfu).

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