BDB - Library Details

Library

Library Name: TAFTSWEEYLDWVGSG fusion M13 phage display library (TAFTSWEEYLDWVGSGX(8,10,12,14,16))

Library ID:

479

Library Length:

8, 10, 12, 14, 16

Library Complexity:

1.3e10

Library Titer:

Library From:

Daniel Kirchhofer (Department of Early Discovery Biochemistry, Genentech, Inc., South San Francisco, California, USA)

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

The libraries were constructed using the standard Kunkel mutagenesis method. The Pep2-8V2A fusion peptide libraries were constructed by fusing randomized peptides via a GSG linker to the C terminus of the anchor peptide Pep2-8V2A (TAFTSWEEYLDWVGSG). The fusion peptide libraries were displayed on the N terminus of M13 major coat protein (p8) following the standard protocol for making phage-displayed libraries. The extension pool consisted of random peptides with 8, 10, 12, 14, and 16 amino acids in length encoded by consecutive degenerate codons (NNK, where N = A/C/G/T and K = G/T). The libraries with different lengths were constructed individually and pooled together at equal concentrations. The final diversity of the library was 1.3e10.

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