BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: pVIII AE(X)3DP phage display library (AEX3DP)

Library ID:

473

Library Length:

Library Complexity:

3.28e4

Library Titer:

Library From:

Junho Chun (Seoul National University College of Medicine,Seoul,Republic of Korea)

Library Randomness:

Completely random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

A restriction endonuclease recognition site (KpnI) was introduced into the sequence encoding the pVIII coat protein leader peptide (LV), using the SnaBI and BamHI restriction enzyme sites, which are located in the VCSM13 genome. Two amino acids (GD) exposed at the N-terminal pVIII coat protein were replaced with random amino acids encoded by NNK degenerate codons (N =A, T,G and C,and K=G and T).

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