BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: CX3CX6CG, CX6CX3CG, CX4CX5CG and CX5CX4CG phage display library pool

Library ID:

468

Library Length:

Library Complexity:

Library Titer:

Library From:

Christian Heinis, Institute of Chemical Sciences and Engineering, Switzerland

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

Peptides are displayed on around five copies of the phage coat protein pIII. The peptide library pool contain peptides of the format ACXmCXnCG (C = cysteine, X = any amino acid). The combinations of ‘m’ and ‘n’ are 3/6, 6/3, 4/5 and 5/4. The genes encoding semi-random peptides, the linker Ser-His-Ser and the two disulfide free domains D1 and D2 were cloned in the correct orientation into the phage vector 21tet.

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