BDB - Library Details

Library

Library Name: X16 and X12 M13 phage display library

Library ID:

464

Library Length:

12 and 16

Library Complexity:

Library Titer:

Library From:

Andreas Ernst and Ivan Dikic (Institute of Biochemistry II, Goethe University, Germany)

Library Randomness:

Completely random

Library Scheme:

Library Topology:

Linear

Comments:

The 16-mer peptide library with 1.0e12 unique peptides was expressed on M13 coat protein p8. The peptide-coding sequences were assembled from trimeric nucleotide building blocks, so that each naturally occurring amino acid (with the exception of cysteine) was encoded with equal probability at each of the 16 amino acid positions. This approach alleviates the risk of introducing unwanted stop codons. The 12-mer peptide library consisted of 1.0e11 unique peptides fused to M13 minor coat protein p3. The peptide-coding sequence was assembled from 12 consecutive repetitions of “NNK” codons (where N represents all four nucleotides and K represents guanine or thymine).

First |

Prev |

464

| Next

| Last