BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: X12 pHEN2 phage display library

Library ID:

461

Library Length:

Library Complexity:

2.0e9

Library Titer:

Library From:

Sangdun Choi (Ajou University, Suwon, South Korea)

Library Randomness:

Completely random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

The 12-mer peptide library was synthesized with forward primer 5'-GCC CAG CCG GCC ATG GCC (NNK)12 TCG AGT GGT GGA GGC GGT TCA G-3' and reverse primer 5'-GCC AGC ATT GAC AGG AGG TTG AG-3'. The two primers were annealed and elongated as described above. Then, the DNA product was digested withNcoI/BamHI and cloned into the pHEN2 phagemid vector with T4 DNA ligase. The library was then transformed into electrocompetent E. coli DH10B cells, generating 2.0e9 distinct clones.

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