BDB - Library Details

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Target
Template
Library
Structure

Library

Library Name: TBAB-cyclized CXnCXnC phage display library (CXnCXnC)

Library ID:

454

Library Length:

9/11/15

Library Complexity:

Library Titer:

Library From:

Christian Heinis (Institute of Chemical Sciences and Engineering, Switzerland)

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

The DNA coding for the random peptide sequences is added to the gene coding for domains D1 and D2 of the cysteine free pIII by PCR using a degenerate primer. The DNA coding for the peptide-D1–D2 fusion is then introduced into the fd-based vector fd0D12 between a leader sequence and the third domain(D3) of pIII. The libraries were generated by cyclizing linear peptides of the format CXnCXnC (C = cysteine, X = random amino acid; n= 3, 4, 6) displayed on the phage with the thiol-reactive reagent N,N′,N″-(benzene-1,3,5-triyl)-tris(2-bromoacetamide) (TBAB).

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