BDB - Library Details

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Template
Library
Structure

Library

Library Name: X10ALLRYX10 phage display library

Library ID:

432

Library Length:

Library Complexity:

Library Titer:

Library From:

Igor Fisch

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

The repertoire of 25 residue "spliced" peptides displayed on phage was created by in vivo recombination between phage and plasmid replicons, followed by self-splicing of the RNA between the two exons (exon 1: NNK10GCTCTCT and exon 2: TAAGGTATNNK10). Each exon comprised a repertoire of 10 random amino acids residues; after splicing, the repertoires were joined together through a central five-residue spacer to give a combinatorial repertoire of 25-residue peptides. The repertoire was displayed on filamentous bacteriophage by fusion to the pIII phage coat protein.

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