BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: CX12C phage display library

Library ID:

429

Library Length:

Library Complexity:

Library Titer:

Library From:

Suk-Jung Choi

Library Randomness:

Completely random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

The linearized pCANTAB5E was ligated with oligonucleotides. Random oligonucleotides are placed between the gene III signal sequence and the E-tag. The E-tag peptide facilitates the identification and purification of a displayed peptide. The randomized sequence of the inserted oligonucleotide consists of twelve NNK triplets where N=A, C, G, or T, and K=G or T. This will produce a fusion protein consisting of a random peptide, E-tag, and gene III protein. The helper phage M13K07 was used to produce a phage library from the bacteria harboring library phagemid.

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