BDB - Library Details

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Target
Template
Library
Structure

Library

Library Name: pZif12 X15 phage display library

Library ID:

424

Library Length:

Library Complexity:

Library Titer:

Library From:

Carl O. Pabo (Massachusetts Institute of Technology, Department of Biology, Cambridge, USA)

Library Randomness:

Completely random

Library Scheme:

Library Topology:

Linear

Comments:

Phagemid vectors used in the selections were created from pZif12 by restoring the reading frame between the Zif12-coding region and gene III and by introducing convenient restriction sites at the start of Zif12. The library containing randomized 15-residue peptides was constructed by cassette mutagenesis, using NN(G/C/T) randomized codons. The complete fusion protein used for phage display contained a PelB signal sequence; a short leader peptide (NH2-EPRAQNS); the random peptide; residues 4–60 of Zif268; a linker that includes an amber codon; and residues 23–424 of M13 gene III product.

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