BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: pComb3.5 X6 phage display library

Library ID:

418

Library Length:

Library Complexity:

Library Titer:

Library From:

Barbas CF 3rd

Library Randomness:

Completely random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

The murine Cys2-His2 zinc finger protein Zif268 was used for construction of phage-display libraries. Primer pairs Aseq (5'-GTCCATAAGATTAGCGGATCC-3') and Zff2r6f [5'-CAGTGTCGAATATGCATGCGTAACTTC(NNK)6ACCACCCACATCCGCACCCAC-3', where N is A, T, G, or C and K is G or T], and Bseq (5'-GTGAGCGAGGAAGCGGAAGAG-3') and Zfnsilb (5'-CATGCATATTCGACACTGGAA-3') were used to amplify fragments of the Zif268 gene using plasmid pAra-Zif268 as a template. The two PCR fragments were used as templates for overlap extension, and the library was constructed in pComb3.5.

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