BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: fUSE5 X5WYA[WF]SPX4 phage dsiplay library

Library ID:

408

Library Length:

Library Complexity:

Library Titer:

Library From:

Landon LA (University of Missouri, Columbia, Missouri, USA)

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

The plus-strand sequence was 5'-TCG GCC GAC GGG GCC (NNK)5 TGG TAT GCG TGG TCC CCG (NNK)4 GGG GCC TCT GGG GCC GAA AGT-3'. The library is based on FUSE5 vector. Random peptides were express on protein III. The resulting peptides contained the six-amino-acid TF antigen–binding consensus sequence (W-Y-A-W/F-S-P) in the conserved central part of the peptide and terminal regions of random amino acid sequence. The large size of the input library (1.0e11 bacteriophage particles) indicated that a large percentage of the possible 15-amino-acid peptides were represented in the library.

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