BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: T7 PXTGTWX8G phage display library

Library ID:

403

Library Length:

Library Complexity:

Library Titer:

Library From:

Watanabe H and Yamasaki K (The Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology)

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

The library was generated by elongating a segment containing an eight-residue randomized region and a glycine residue Xaa8-Gly at the C terminus of a chignolin-derived segment termed CLN. Here, the Xaa was encoded by a degenerate codon NNK. The gene fragments were synthesized by overlap extension polymerase chain reaction (PCR), digested by restriction enzymes EcoRI/HindIII, and then ligated into the C-terminal part of a T7 phage coat protein gene 10 (g10) of the T7Select 10 -3b vector (Novagen).

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