BDB - Library Details

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Library

Library Name: T7 CX9C phage display library

Library ID:

390

Library Length:

Library Complexity:

Library Titer:

Library From:

Richard G. Smith (University of Nottingham, Cancer Research Laboratories, School of Pharmaceutical Sciences, Nottingham NG7 2RD, UK)

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

The T7Select Phage Display System immunology utilizing the T7Select415-1b vector (Novagen, Inc., Madison, WI, USA), was used to display approximately 415 identical copies of each peptide on the capsid head. The peptides were encoded for by a degenerate oligonucleotide insert, consisting of a randomized library of 9-residue peptides flanked by cysteine residues (to impose a structural constraint on the C-terminus of the displayed peptides). These cysteine were also flanked with serine residues, such that the N-terminus of the peptide linked to the capsid head through a serine residue.

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