BDB - Library Details

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Target
Template
Library
Structure

Library

Library Name: CX8C fUSE5 phage display library

Library ID:

356

Library Length:

Library Complexity:

Library Titer:

5.0e8

Library From:

Piero Pollesello (Orion Pharma, R&D)

Library Randomness:

Completely random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

Briefly, the RF form of the fUSE5 vector of fd phage was prepared by transfecting fUSE5 DNA into E. coli K802 cells. The inserts were prepared by synthesizing degenerate 82-bases-long DNA oligonucleotides containing the sequence: 5\'-CTATTCTCACTCGGCCGACGGGGCTTGCNNT⁄GNNT⁄GNNT⁄GNNT⁄GNNT⁄GNNT⁄GNNT⁄GNNT⁄GTGCGGGGCCGCTGGGGCCGAAACTGTTGAA-3\', and they were used as target molecules in the following PCR reactions. These target sequences were amplified by PCR using synthetic DNA oligonucleotides: 5\'-CTATTCTCACTCGGCCGACG-3\' (5\'-end primer) and 5\'-TTCAACAGTTTCGGCCCCAG-3\' (3\'-end primer).

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