BDB - Library Details

Library

Library Name: CX9C E. coli bacterial display library

Library ID:

332

Library Length:

Library Complexity:

Library Titer:

Library From:

Sang J. Chung

Library Randomness:

Completely random

Library Scheme:

NNN

Library Topology:

Linear

Comments:

The FcBP1-eGFP vector, which encodes a fusion protein of eGFP with FcBP1, at the C-terminus of FcBP1, was newly designed and constructed by PCR. An FcBP-eGFP DNA library was constructed by inserting 27 nt-randomized DNA sequences into the N-terminus of the eGFP gene by PCR using the eGFP gene as template under the conditions described above (50-primer: 50-GCC GCA GCC ATA TGA AAG ATG AAT GTN NNN NNN NNN NNN NNN NNN NNN NNN NNT GCG AAT TCG AGC TCC GTC GA-30; 30-primer, ATT CGC GGA TCC CTT GTA CAG CTC GTC CAT GCC GAG; where N is A, T, G, or C, and the restriction sites for NdeI and BamHI are italicized). The purified PCR product was digested with NdeI and BamHI and cloned into pHCE-IIB, yielding the FcBP-eGFP DNA library.

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