BDB - Library Details

Library

Library Name: IR20 phage display library (X20)

Library ID:

294

Library Length:

Library Complexity:

4.0e7

Library Titer:

Library From:

Kathlynn C. Brown

Library Randomness:

Completely random

Library Scheme:

NNS

Library Topology:

Linear

Comments:

A new library of random 20-mer peptides in a phage vector was created by cloning the sequence into bacteriophage vector fAFF-1. A library of 4.0e7 independent clones encoding 20-mer peptide sequences embedded between the BspEI and Acc65I cloning sites of fAFF-BA was constructed. This 20-mer library, referred to as IR20, differs from the original library in that the cloning region within the bacteriophage vector has been modified. This allows for efficient directional cloning and results in the addition of Ser-Gly to the N-terminus of the peptide-pIII fusion. In the previously used library, the 20-mer library is located directly at the N-terminus of the fusion, and it is possible that modification of the N-terminal amino group of the peptides may disrupt cell targeting.

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