BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: JCFN-RGD phage display library (XRGDXXXX)

Library ID:

268

Library Length:

Library Complexity:

1.5e9

Library Titer:

Library From:

Library Randomness:

Semi-random

Library Scheme:

NNK

Library Topology:

Linear

Comments:

The phage-display vector for FNfn10, JCFN, was constructed by cloning the FNfn10 gene7 in the modified M13 vector, JC-M13-88. A FNfn10 library, JCFN-RGD, was constructed in such a way that the FG loop has XRGDXXXX sequence where X stands for any amino acid (residues 77–84). Mutagenesis on the JCFN template was performed using an oligonucleotide JCFNFGRGD (GTTAATCGAGATTGGCTTGGAMNNMNNMNNMNNATCGCCGCGMNNAGTAACAGCGTATAC, where N is a mixture of A, G, C and T, and M is a mixture of A and C).

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