BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: C9C T7 phage display library

Library ID:

227

Library Length:

Library Complexity:

Library Titer:

1.0e10

Library From:

I. Todd

Library Randomness:

Completely random

Library Scheme:

Library Topology:

Circular

Comments:

The peptides were expressed near the C-terminus of the T7 gene X surface coat protein (415 copies per phage). The random peptides of the T7 library were encoded by double stranded DNA inserts assembled from synthetic degenerate oligonucleotides and cloned into gene X of the vector (T7select415-1) (Bioscience, Cambridge, UK) at HindIII and EcoRI restriction sites. The vector DNA and insert DNA were ligated with T4 DNA ligase, and assembled into phage using T7Select packaging extract (BioScience, Cambridge, UK). The phage were amplified in E. coli BL21.

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