BDB - Library Details

Library

Library Name: R8C phage display library

Library ID:

215

Library Length:

Library Complexity:

1.1e8

Library Titer:

Library From:

Heather Hanson Pierce

Library Randomness:

Completely random

Library Scheme:

Library Topology:

Linear

Comments:

Two oligonucleotides were synthesized to generate the DNA cassette used in the construction of the conformationally constrained library. The first was a degenerate oligonucleotide, 5'-TGACGTCTCGAGTTGT(NNK)8-TGTGGATCTAGAAGGATC-3', containing an Xho I site, where N represents an equimolar mixture of A, C, G and T; and K corresponds to an equimolar mixture of G and T. The NNK coding scheme utilizes 32 codons to encode 20 amino acids; the frequency of each amino acid is once (Cys, Asp, Glu, Phe, His, Ile, Lys, Met, Asn, Gln, Trp, Tyr), twice (Ala, Gly, Pro, Val, Thr), or thrice (Leu, Arg, Ser) per codon. The second oligonucleotide, 3'-CCTAGATCTTCCTAG-5', contained an Xba I site, and served as a primer for DNA synthesis.

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