BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: X14 and CX14C phage display library pool

Library ID:

117

Library Length:

Library Complexity:

Library Titer:

Library From:

Anthony N. Hodder

Library Randomness:

Completely random

Library Scheme:

Library Topology:

Comments:

The pool consists two 14-residue libraries displaying peptides on M13 gene VIII coat protein. One is a linear library with all 14 residues randomized. Another is a cyclic library with all 14 residues randomized in the context of a pair of fixed cysteine residues. In the cyclic library, the spacing of the cysteine residues varied from three to six residues. The randomization of residues for both libraries was achieved using Kunkel mutagenesis, employing degenerate oligonucleotides in which each randomized residue was encoded by an NNS codon. Equal aliquots of each linear and cyclic sublibrary were combined, giving rise to the 14-residue pool for panning.

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