BDB - Target Details

Target

Target Name: In vitro M cell coculture system

Target ID:

848

Target Type:

Cell

Target Sequence:

Synonyms:

Target Source:

Unknown

Target Structure:

Comments:

A human colon carcinoma cell line, Caco-2, and a human Burkitt's lymphoma cell line, Raji, were purchased from the American Type Culture Collection (Manassas, VA). The in vitro M cell coculture model was assembled as described by Kerne′is et al. and modified by Gullberg et al. Briefiy, 0.1 and 1.5 ml DMEM supplemented with 10% FBS were added to the apical and basolateral sides of the transwell, respectively, and the transwells then were preincubated for 30 min in a CO2 incubator. The Caco-2 cells then were spread to the apical side of the transwell. After incubation for 3 h, the medium at the apical side was changed, and the upper medium was changed every other day for the next 14 d of culture. Raji cells suspended in RPMI 1640/DMEM (1:2) mixture then were added to the basolateral chamber of the transwell, and the coculture was maintained for 2 d. Caco-2 cells cultured alone by this procedure, without the Raji cells, served as a control.

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