BDB - Target Details

Target

Target Name: Cystic fibrosis mucus model

Target ID:

2130

Target Type:

Miscellaneous

Target Sequence:

Synonyms:

Target Source:

Unknown

Target Structure:

Comments:

The cystic fibrosis (CF) mucus model was prepared according to literature reports of composition to model sputum of CF patients. The model is an aqueous mixture of 40 mg/mL mucin from porcine stomach type III (Sigma Aldrich, MO; catalog number M1778), 1.4 mg/mL herring sperm DNA (Promega, WI; catalog number D1811), 5 mg/mL egg yolk from chicken (Sigma Aldrich, MO; catalog number E0625), 0.9 mg/mL lactoferrin human (Sigma Aldrich, MO; catalog number L4040), 5 mg/mL sodium chloride (Thermo Fisher Scientific, MA; catalog number S271-500), and 2.2 mg/ mL potassium chloride (Thermo Fisher Scientific, MA; catalog number P217-500) to give the concentration of ions found in CF sputum. The solution was adjusted with 0.5 M hydrogen chloride (Thermo Fisher Scientific, MA; catalog number AC423795000) to pH 6.5, which is the estimated pH of CF airway mucus. The reagents were transferred to a sterile tube, mixed for 2 h in a tube rotator at 5 rpm (Thermo Fisher Scientific, MA), and used within 4 h.

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