BDB - Target Details

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Target

Target Name: Soluble proteins differentially expressed between the control and dimethylhydrazine treated groups

Target ID:

2106

Target Type:

Miscellaneous

Target Sequence:

Synonyms:

Target Source:

Rattus norvegicus (Rat):10116

Target Structure:

Comments:

Protein extraction for the phage display technique was performed from samples of large intestine tissue from Wistar rats with chemically induced colon-rectal tumors. These tumor tissue samples were donated by Silva et al. and treated as follows. Briefly, 50 mg of the tissue was macerated with liquid nitrogen and suspended in 1 mL of 20 mM Tris–HCl pH 7 buffer, 250 mM sucrose, and protease inhibitor cocktail (PIC). The samples were sonicated and centrifuged at 20,000 ×g, 4°C for 30 min.

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