BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 976

Peptides:

Sequences

Affinity Values

PMPHSLNFSQYLWYT(11)
MHRSLWEWYVPNQSA(9)
HTYSSLWDTYSPLAF(8)
SQTGTLNTLFWNTLR(8)
WHPGLSFGSYLWSKT(6)
SSLWTRYAWPSMPSY(5)
TLFMDLWHDKHILLT(4)
ILNFPLWHEPLWSTE(2)
WSFYNLHLPEPQTIF(2)
PLDLWSLYSLPPLAM(2)
LDLWMRHYPLSFSNR(1)
PALLNWSFFFNPGLH(1)
PTLWQLYQFPLRLSG(1)
ISFSELMWLRSTPAF(1)
LSEADLWTTWFGMGS(1)
SSLWRIFSPSALMMS(1)
SLPTLTSILWGKESV(1)
IKTDEKMGLWDLYSM(1)
SHIKSLLDSSTWFLP(1)

[0.01]
[>23]
[0.34]
[10.0]
[0.40]
[0.40]
[8.0]
[9.0]
[1.0]
[2.0]
[0.38]
[1.0]
[2.5]
[5.0]
[7.0]
[8.0]
[8.0]
[23.0]
[>47]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8041758

Target Name:

Urokinase plasminogen activator surface receptor

Template Name:

Urokinase-type plasminogen activator (uPA)

Structure of Target-Template Complex:

2FD6

2I9B

3BT1

3BT2

3U73

4K24

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 M13 phage display library

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

The IC50 (μM) was determined and shown. Each of them showed 20- to 500-fold greater yields than an irrelevant bacteriophage.

Brief Description:

First round, uPAR-expressing S59 cells were used; Sf9 insect cells expressing the human substance P receptor were used to remove nonspecifically adherent bacteriophage. Second round, Eluted, amplified bacteriophage were affinity selected on transfected COS-7 cells overexpressing human uPAR. Third round, affinity selection was done on uPAR-expressing S59 cells again.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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