BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 970

Peptides:

Sequences
RPHQVV(1) LPFNMT(1) VPRAIS(1) EAFGMR(1) RPQTLA(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7896777

Target Name:

Stromelysin-1, SL-1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X6 fAFF1-tether C phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the first round, 2E11 TU (about 1000 equivalents of each clone) were treated with stromelysin. The output was not titered before the next round of screening, thus variable amounts of phage were used as input for the succeeding round. In later rounds of screening, the protease concentration was reduced. After each round, 12 clones were analyzed using the phage proteolysis assay.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

970

| Next

| Last