BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 953

Peptides:

Sequences

Affinity Values

EHDFHHIREWGNHWKNFLAVM
YEKPWQNLWDWGAEAFKDLLDK
LSFDWSELRRWGTWAAAEVFEL
ELWRNLRLWGYCMNLSNMPL
EFSLFKDIYRWGNWAAGFYYGV
SFCLFDSIFDWGARGAEWHVG
SCNMWRNIYSWGATFPQDHIFF
DMDLFQHLSCWGTDFADMMFDE
SCAWFLHLRQWGNSNWTYIFGV
MHFGWYHLSEWGKIAALIGFEM
ETGWFHNLTAWGEESVYALHIM
SPGLFDNLKTWGTRMEHFMSLS
QSFSMMSRWGQEFHNMLINV
TALGWVHMHEWGEEFSKFPWGN
QDYWTNMLIWGSMLLWSLFGADP
HDYWQSMNHWGGVYGGCVPMCK
YEEMWQWVFKAGQEWPWSYFPM
QIPFQIQAYWLDVCCQEVTDDL
IDQMWQWDPRWGXXXEEIDIHD
SAFEYIQLTVSGWLPHLMGLF
EKFEILCINYQGWISDLCEAIA
WHDEDVLFRNLGEATLWLAEYY
DWECMYDFCWRGXCVALNSTG
DQTYKFEYNMGKDVTSLVCYW
DNWIDSLVCIVGTGYGSLAAYY

[150 ± 30]
[240 ± 80]
[150 ± 40]
[930 ± 130]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

[WF]-x-[LIM]-x-W

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7542403

Target Name:

Antigen-presenting glycoprotein CD1d1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X11GX12 phage display library

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

Dissociation constants (nM) for unlabeled peptides were determined from the molar concentration giving 50% inhibition of 125I-p99 binding to mCD1 in a competitive binding assay. NT denotes not tested.

Brief Description:

The recombinant soluble CD1-beta2 microglobulin complexes (mCD1) expressed in Drosophila melanogaster SC2 cells were used in the panning. The mCD1 was also engineered with a COOH-terminal hemagglutinin (HA) tag (YPYDVPDYAS), an epitope derived from the influenza HA protein. In this way, mCDl-phage complexes could be identified with a HA tag-specific antibody.

BiopanningDataSet Comments:

Forty-seven different clones were selected by mCD1 binding. However, only 25 sequences shows a well-defined core motif were given in the original paper.

Curator:

Curation Date:

0000-00-00

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