BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 894

Peptides:

Sequences

MDHLGPGRYHWFNRNDRVFK(4)
GVRHMGPGRASFDEFQSLHV(3)
WVFSPSTGGHRLYTGPGRHF(2)
RFMSAYVMKIGPNRHGFRAP(2)
RSQGLMVLGPGRGFMIFNAG(2)
RRWCRSPNNLFIASPHGPLR(1)
AAPAGVLHIRHLVGPNRYNF(1)
NLRRTSIRAFYMLGPSRWMP(1)
VRKPFQFAASLGPHRAFSHW(1)
RAKWNKHLYFEGPSRMFNFT(1)
SLVPSPLLYIGPGRLRMTDH(1)
ASRYEVFPLLGPHRMMFKFP(1)
TEVWSRFWIGPARSLGVDQS(1)
FIGGNYKLGRGRANFVNYAT(1)
SQLHWRGPGRTRFVSDRFH(1)
AASNEFLGAGRLNFDKPKHS(1)
RKDGLIGPGRGGFQGMRWVM(1)
GRYLLGPGRELVSLVRPAMH(1)
RIVLGPARFFFPQSYLLDQG(1)

Sequences Features:

Motif:

[YL]-[VLI]-G-P-G-R-x-F

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7506691

Target Name:

Anti-HIV-l isotype MN gp120 monoclonal antibody 58.2

Template Name:

Surface protein gp120 of Envelope glycoprotein gp160

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

PDL-20 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Micropanning protocol: the mAB was diluted to 50 pg/ml in 0.1M NaHCO3, buffer, pH 9.2, and 50pl added to each well of a Nunc Maxisorb microtiter plate. The mAb was bound to the plate for 2h at room temperature, the wells washed with PBS and blocked with 1% powdered milk in PBS for 1h at room temperature. After blocking, the wells were washed four times with PBS, the phage added in 50pl of milk/PBS and bound to the mAb for 1 h at room temperature. The plates were washed 12 times with 0.5% Tween20 in PBS to remove non-specific phage and the phage were eluted by treatment with 50pl of 0.1M HCl adjusted to pH 2.2 with glycine. Eluted phage were immediately neutralized with 10ul of 1M Tris base.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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