BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 882

Peptides:

Sequences	Affinity Values
RSLRPLPPLPLPPRH(9) RSLRPLPPLPLPPRT(1) RSLRPLPPLPLPPRL(1) RSLRPLPPLPLPPPH(1)	[477.953] [357.737] [NT] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7479908

Target Name:

PI3K SH3 domain of PI3-kinase subunit p85-alpha

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

C+5 class I phage display library

Affinity Measurement Method:

Surface plasmon resonance (SPR)

Affinity Measurement Description:

Binding of phage-displayed peptides to Src was measured by BIAcore technology. The amount of phage binding to immobilized SH3 domain is given as arbitrary resonance units (RU) and has been corrected for bulk refractive-index contributions of the unbound phage. Data shown were reproduced from the graph. NT denotes not tested.

Brief Description:

GST-PI3K SH3 was produced in Escherichia coli strain BL21, purified and immobilized on polystyrene as the target.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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