BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 858

Peptides:

Sequences
DFLAWL(14) DFLEML(2)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 1696028

Target Name:

Anti-Myohemerythrin monoclonal antibody A2

Template Name:

Myohemerythrin, MHr

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-6mer phage display library (X6)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the first round, 1e12 phage particles were incubated overnight with 1000nM bio-MAb in 10 microliter reaction mixtures; the phages were diluted and reacted 10 to 20 minutes on streptavidin-coated dishes; the dishes were extensively washed; and the bound phages were eluted in acid buffer. Phages eluted in the first round were amplified on agar medium and subjected to second round of paning with the same bio-MAb at concentrations of 0.1 nM. Phages from the second round were amplified and subjected to third round with 0.1 nM bio-MAb.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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