BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 855

Peptides:

Sequences

Affinity Values

KAAQIYSEP(8)
DYDGYGWRE(3)
YAADITNGL(3)
NKPNWDGYA(1)
IQWDGYARQ(1)
WSSGYGTGR(1)
AYESGYNLP(1)
CSAGICFSE(1)

[0.684, 20.0 ± 6.0]
[0.360, 9.3 ± 0.2]
[0.648, 11.0 ± 2.3]
[0.648, 10.1 ± 0.2]
[0.432, ND]
[0.234, 8.2 ± 1.0]
[0.252, 9.7 ± 1.5]
[0.414, 7.2 ± 1.6]

Sequences Features:

Motif:

[DS]-G-Y-[AG], A(2)-A-x-I

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10759860

Target Name:

Anti-CA monoclonal antibody A-56.36

Template Name:

Crotoxin acid chain, CA

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X9 and CX9C phage display library pool

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

ELISA measurements of the binding to A-56.36 expressed as A490, SD ± 10%. ELISA measurements of the competition with CA, expressed as the inhibition percentage of the binding of A-56.36 to CA-coated plates in the presence of a fixed amount of phages (e12 p.f.u./mL). Average values and SD on two experiments are shown. ND denotes not determined.

Brief Description:

BiopanningDataSet Comments:

By screening random phage-displayed libraries with the mAb, phagotopes bearing the (D/S)GY(A/G) or AAXI consensus motifs were selected. They all bound A-56.36 in ELISA and competed with CA for mAb binding, although with different reactivities.

Curator:

Curation Date:

0000-00-00

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