BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 830

Peptides:

Sequences	Affinity Values
GEASGLCCRWSSLKGC(30) GHREAFLPPWVFXFL(11) GEASGLCCRWSLRVV(8) YFTIPATLLPFGV(1) GNRPVRPQVYFSASS(1) SGFVAGKGFRLDFGS(1)	[NA] [NA] [NA] [NA] [82%] [88%]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10649627

Target Name:

Anti-MAPS monoclonal antibody 9C10

Template Name:

Meningococcal group A polysaccharide (MAPS)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-15mer phage display library (X15)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Six phage clones were screened for MAPS mimicry by their ability to inhibit binding of human hyperimmune sera to nominal antigen, MAPS, by inhibition ELISA. The results of the inhibition ELISA with phage clone six (SGFVAGKGFRLDFGS) indicate that 5.5e10 TU of phage clone 6 inhibited the binding of human hyperimmune sera to MAPS (88% inhibition). The control phage, containing no insert, failed to inhibit binding in this assay. Phage clone 4 (GNRPVRPQVYFSASS) was also capable of inhibiting the binding of human hyperimmune sera to MAPS (82% inhibition). Other phage clone failed to inhibit binding of human hyperimmune sera to MAPS (data not shown).

Brief Description:

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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