BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 798

Peptides:

Sequences

Affinity Values

DLETSFMKV(3)
DLVTSKLQV(1)
DLETSFMPA(1)
DLQTEKYMG(1)
DLMTSKYMA(1)
DLETKFMRQ(1)
KDLLTTKLS(1)
KDLQTRAMH(1)
KDLATSKLL(1)
RDLSTRPLW(1)
RDLLTSKYV(1)
TDLQTMAMR(1)
NLDLHTEKI(1)
RAGDLTTTK(1)
ELNTWKMDW(1)
DPESSFMKV(1)
NPRLETYAM(1)
RALSPPPMS(1)
SSRLETSYL(1)
ENLSNWQIS(1)

[NT]
[0.76 ± 0.02]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10029604

Target Name:

Human neutrophil cells

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

J404 phage display library (X9)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Purified phage clones were incubated in microtiter plates coated with PMN freshly isolated from peripheral venous blood. Bound phages were detected with polyclonal anti-phage antibody and quantified by light absorbance. Optical density values, OD (405 nm), are representative of one of two experiments showing the mean ± SD of quadruplicate determinations. NT represents not tested.

Brief Description:

Freshly isolated PMN were incubated with 20 μL aliquots of phage library in 1 mL HBSS containing 0.1% bovine serum albumin (phage buffer) for 2 hours at 4°C in a 1.5 mL microcentrifuge tube. PMN were then washed five times with phage buffer and bound phage were eluted for 5 minutes in 2 mL of 0.1 mol/L glycine (pH 2.2) followed by addition of phage buffer containing 0.5% Tween 20 to the remaining cell pellet.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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