BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 777

Peptides:

Sequences

Affinity Values

YSPFSW
YVSWSPD
FSWSDT
YSWSDM
WSPFPS
DSPFSF
FSPFDW
TSPFPW
YSFFPW
YSDFPW
DSWFPW
SSFYSS
DSWSTS
FSPFSF
SSPFDW

[37, 110]
[35, 100]
[19, 90]
[10, 60]
[26, 60]
[22, 80]
[21, 90]
[31, 90]
[16, 70]
[26, 60]
[14, 20]
[22, 90]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10547288

Target Name:

Protein Nef

Template Name:

Tyrosine-protein kinase HCK

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

RRT-SH3 phage display library

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

The relative potency of the corresponding SH3 domains to compete with labeled Hck-SH3 Nef and RRT.A1 Nef in binding to Nef, respectively, expressed as probe:competitor ratio at 50% inhibition. NT denotes not tested.

Brief Description:

BiopanningDataSet Comments:

To examine if it would be possible to present functional SH3 domains on the surface of bacteriophages authors constructed a phagemid containing Hck-SH3, and by using a M13KO7 helper virus produced recombinant phages expressing it fused to the pIII coat protein.

Curator:

Curation Date:

0000-00-00

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