BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 759

Peptides:

Sequences	Affinity Values
IEAYAKKRK(2) GHKVKRPKG(2) KNKIPKSPK(1)	[1.78] [1.94] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11070036

Target Name:

Transferrin receptor protein 1

Template Name:

Iron-loaded human transferrin

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X9 phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance at 490 nm was measured. Data shown were repeoduced from the graph and expressed as the means ± SEM. NT denotes not tested.

Brief Description:

Bound phage were eluted with low-acid buffer (0.1 M glycine, pH 2.2) and ligand (iron-loaded human transferrin; Sigma-Aldrich) in TBS buffer (50 mM Tris-Cl, pH 7.5; 150 mM NaCl).

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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